Voltammetric Quantification of Adenine and Guanine at C60 Modified Glassy Carbon Electrodes

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Abstract
Voltammetric determination of adenine and guanine has been carried out at C60 modified glassy carbon electrode at physiological pH. Well-defined oxidation peaks were observed with E p at ∼990 mV and 692 mV for adenine and guanine respectively. Good colinearity was obtained in the concentration range 0.5 μM to 100.0 μM for adenine and guanine with sensitivity of ∼0.06 μA μM−1 and ∼0.03 μA μM−1, respectively. Simultaneous voltammetric determination of adenine and guanine has been described. Recovery studies for adenine and guanine in biological samples were also carried out. Interfering effect of some common metabolites including ascorbic acid has been evaluated. Analytical application of the developed protocol for determination of (G + C)/(A + T) ratio in DNA sample is also described.