Solubilization of Brain Benzodiazepine Receptors with a Zwitterionic Detergent: Optimal Preservation of Their Functional Interaction with the GABA Receptors
Authors:
- Angel L. de BlasABAngel L. de Blas
- 1 March 1983
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 41 (3) , 752-758
Abstract
Rat brian benzodiazepine receptors were solubilized by means of the zwitterionic detergent CHAPS [3-[(3-chlorodipropyl)dimethylammonio]-1-propane sulfonate] under conditions in which the GABA stimulation of [3H]flunitrazepam binding to the benzodiazepine receptors is maintained intact. This stimulation is partially or totally abolished when using other conventional detergents.Keywords
This publication has 32 references indexed in Scilit:
- Soluble gamma-aminobutyric acid and benzodiazepine receptors from rat cerebral cortexLife Sciences, 1981
- Differential ontogeny of Type 1 and Type 2 benzodiazepine receptorsLife Sciences, 1981
- Soluble benzodiazepine receptors: Gabaergic regulationLife Sciences, 1980
- Putative benzodiazepine receptor: A protein solubilised from brainFEBS Letters, 1979
- Solubilization of histamine H-1, GABA and benzodiazepine receptorsLife Sciences, 1979
- Solubilization of benzodiazepine binding site from rat cortexLife Sciences, 1979
- Molecular Mechanisms in the Receptor Action of BenzodiazepinesAnnual Review of Pharmacology and Toxicology, 1979
- Is the benzodiazepine receptor coupled to a chloride anion channel?Nature, 1979
- GABAergic modulation of benzodiazepine binding site sensitivityNature, 1978
- Benzodiazepine receptors in rat brainNature, 1977