Detection and quantification ofBifidobacterium lactisLAFTI�B94 in human faecal samples from a consumption trial
Authors:
- Ping SuPSPing Su
- Anders HenrikssonAHAnders Henriksson
- Jade E. TandianusJTJade E. Tandianus
- Ji Hyeon ParkJPJi Hyeon Park
- Frances FoongFFFrances Foong
- Noel W. DunnNDNoel W. Dunn
- 1 March 2005
- journal article
- clinical trial
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 244 (1) , 99-103
Abstract
A method was developed to allow detection of the probiotic Bifidobacterium lactis LAFTI B94 in human clinical samples. A new probe, Laf94p, was developed to accomplish colony hybridization of B. lactis B94. PCR detection of B94 was also achieved using the species-specific (B. lactis) primer pair. These tests and probes allowed detection and quantification of B94 in the human intestinal flora. The sensitivity of the probe was assessed by monitoring faecal levels of B94 in humans who were fed the culture. In this trial, five volunteers were fed with the probiotic. The presence of B94 was assessed daily. Viable B94 could be detected at high levels (as high as 1.8 x 10(9) cfu g(-1) wet weight) during the feeding period. Four weeks after the feeding stopped, B94 could still be detected in one subject. These results indicate that B94 survives in the human gastrointestinal tract.Keywords
This publication has 8 references indexed in Scilit:
- Role of probiotics in the modulation of intestinal infections and inflammationCurrent Opinion in Gastroenterology, 2004
- Probiotics: an overview of beneficial effectsAntonie van Leeuwenhoek, 2002
- Specific Identification and Targeted Characterization of Bifidobacterium lactis from Different Environmental Isolates by a Combined Multiplex-PCR ApproachApplied and Environmental Microbiology, 2001
- Selection of a Bifidobacterium strain to complement resistant starch in a synbiotic yoghurtJournal of Applied Microbiology, 2001
- Probiotics: effects on immunityThe American Journal of Clinical Nutrition, 2001
- Identification and quantification of Bifidobacterium species isolated from food with genus-specific 16S rRNA-targeted probes by colony hybridization and PCRApplied and Environmental Microbiology, 1997
- Phylogenetic identification and in situ detection of individual microbial cells without cultivation.Microbiological Reviews, 1995
- Gene organization and primary structure of a ribosomal RNA operon from Escherichia coliJournal of Molecular Biology, 1981