Processing and secretion of the N‐terminal domain of α‐dystroglycan in cell culture media
Authors:
- Kiichiro MatsumuraKMKiichiro Matsumura
- 15 January 2008
- journal article
- Published by Wiley in FEBS Letters
- Vol. 582 (3) , 439-444
Abstract
α‐Dystroglycan (α‐DG) plays a crucial role in maintaining the stability of muscle cell membrane. Although it has been shown that the N‐terminal domain of α‐DG (α‐DG‐N) is cleaved by a proprotein convertase, its physiological significance remains unclear. We show here that native α‐DG‐N is secreted by a wide variety of cultured cells into the culture media. The secreted α‐DG‐N was both N‐ and O‐glycosylated. Finally, a small amount of α‐DG‐N was detectable in the normal human serum. These observations indicate that the cleavage of α‐DG‐N is a widespread event and suggest that the secreted α‐DG‐N might be transported via systemic circulation in vivo.Keywords
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