Development of a Deep Learning Model to Estimate Anemia from Palpebral Conjunctiva Taken with a Portable Slit-Lamp Microscope

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Abstract
Background: Anemia is a common systemic condition associated with adverse maternal, perioperative, and cardiovascular outcomes. Although timely screening is clinically important, diagnosis still relies on invasive blood testing. Palpebral conjunctival pallor has traditionally been used as a noninvasive indicator of anemia, but its diagnostic accuracy remains limited. This study aimed to develop and validate a deep learning system to estimate hemoglobin (Hb) concentration and screen for anemia using palpebral conjunctiva images captured with a smartphone-compatible slit-lamp microscope. Methods: In this prospective observational study, 225 Japanese participants (20–92 years) underwent conjunctival imaging and blood testing. Palpebral conjunctiva videos were obtained using the Smart Eye Camera. Video frames were processed using automated anterior-segment segmentation and conjunctiva extraction. A ConvNeXt-based regression model was trained to predict Hb values. Anemia was defined using sex-specific Hb thresholds. Results: From 225 videos, 53,776 frames were extracted, yielding 9903 quality-filtered conjunctiva images (training: 8082; test: 1821). Video-level predicted Hb values moderately correlated with measured Hb (r = 0.42). For anemia screening, frame-level analysis achieved an AUC of 0.75, with accuracy of 0.76, sensitivity of 0.71, and specificity of 0.79. Video-level aggregation achieved 69% accuracy. Conclusions: Deep learning analysis of palpebral conjunctiva images acquired with a portable slit-lamp microscope demonstrated the feasibility of non-invasive hemoglobin estimation and anemia screening. Although the proposed approach achieved moderate performance, further improvements in model accuracy and prospective multi-center validation are required before clinical implementation.
Funding Information
  • Japan Agency for Medical Research and Development
  • Japan Science and Technology Agency
  • Japan Society for the Promotion of Science (JSPS) Kakenhi
  • New Energy and Industrial Technology Development Organization

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