Effects of the Inclusion of Equex STM into Tris‐Based Extender on the Motility of Dog Spermatozoa Incubated at 5°C

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Abstract
Contents: The aim of this study was to estimate the effects of Equex STM on sperm motion characteristics in chilled dog semen extended in Tris‐based diluent. Thirty‐two ejaculates were collected from 12 proven German shepherd stud dogs. The sperm‐rich fractions were diluted in Tris‐based extender with 1% (v/v) Equex STM (sample A) and in Tris‐based extender with no addition of detergent (sample B). The extended semen was incubated for 240 h at 5°C and the motility parameters were evaluated by CASA system at 24‐h intervals. Addition of Equex STM to Tris‐based extender led to an initial activation of motion activity of spermatozoa, followed by a rapid decrease, shortening the lifespan of spermatozoa incubated at 5°C. Computer‐assisted sperm analysis clearly showed that Equex STM‐induced changes of sperm motion characteristics resemble the hyperactivation (HA) of spermatozoa associated with the capacitation process.

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