Detection of parallel and antiparallel DNA triplex structures in living human cells using in-cell NMR
- 7 June 2021
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Chemical Communications
- Vol. 57 (52) , 6364-6367
Abstract
We introduced oligodeoxynucleotides (ODNs) that form parallel and antiparallel triplex structures in vitro into living human cells and recorded their in-cell NMR spectra. Observation of landmark signals for triplex structures proved for the first time that parallel and antiparallel triplex structures are formed in living human cells.Keywords
Funding Information
- Japan Agency for Medical Research and Development (JP20fk0410027)
- Japan Society for the Promotion of Science (17H05878, 17K14515, 18H04550, 19K16054, 20H03192, 20K06524, 20K21477)
- Ministry of Education, Culture, Sports, Science and Technology
This publication has 32 references indexed in Scilit:
- A Gel-Encapsulated Bioreactor System for NMR Studies of Protein-Protein Interactions in Living Mammalian CellsAngewandte Chemie, 2012
- A Resealed-Cell System for Analyzing Pathogenic Intracellular Events: Perturbation of Endocytic Pathways under Diabetic ConditionsPLOS ONE, 2012
- Observation of NMR Signals from Proteins Introduced into Living Mammalian Cells by Reversible Membrane Permeabilization Using a Pore-Forming Toxin, Streptolysin OJournal of the American Chemical Society, 2009
- DNA triple helices: Biological consequences and therapeutic potentialBiochimie, 2008
- Comparison of triple helical COMBO-FISH and standard FISH by means of quantitative microscopic image analysis of abl/bcr positions in cell nucleiJournal of Biochemical and Biophysical Methods, 2007
- Triplex DNA and human diseaseFrontiers in Bioscience-Landmark, 2007
- Solution structure of a purine·purine·pyrimidine DNA triplex containing G·GC and T·AT triplesStructure, 1993
- Environmental influences on the in vivo level of intramolecular triplex DNA in Escherichia coliBiochemistry, 1993
- Proton nuclear magnetic resonance assignments and structural characterization of an intramolecular DNA triplexJournal of Molecular Biology, 1992
- Protonated triplex DNA in E. coli cells as detected by chemical probingFEBS Letters, 1990