Decoding the olfactory map through targeted transcriptomics links murine olfactory receptors to glomeruli
Open Access
- 1 September 2022
- journal article
- research article
- Published by Springer Nature in Nature Communications
- Vol. 13 (1) , 1-18
Abstract
Sensory processing in olfactory systems is organized across olfactory bulb glomeruli, wherein axons of peripheral sensory neurons expressing the same olfactory receptor co-terminate to transmit receptor-specific activity to central neurons. Understanding how receptors map to glomeruli is therefore critical to understanding olfaction. High-throughput spatial transcriptomics is a rapidly advancing field, but low-abundance olfactory receptor expression within glomeruli has previously precluded high-throughput mapping of receptors to glomeruli in the mouse. Here we combined sequential sectioning along the anteroposterior, dorsoventral, and mediolateral axes with target capture enrichment sequencing to overcome low-abundance target expression. This strategy allowed us to spatially map 86% of olfactory receptors across the olfactory bulb and uncover a relationship between OR sequence and glomerular position.Keywords
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Funding Information
- U.S. Department of Health & Human Services | NIH | National Institute on Deafness and Other Communication Disorders (F31DC017394, K99DC018333, R01DC016224, R01DC014423)
- U.S. Department of Health & Human Services | NIH | National Institute of Mental Health (F32MH115448)
- U.S. Department of Health & Human Services | NIH | National Institute on Deafness and Other Communication Disorders
- National Science Foundation (1555919)
- U.S. Department of Health & Human Services | NIH | National Institute on Deafness and Other Communication Disorders
- U.S. Department of Health & Human Services | NIH | National Institute on Deafness and Other Communication Disorders
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