Antimicrobial potential and biological properties of modified glass-ionomer cement supplemented with chlorhexidine diacetate

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Abstract
Purpose: This study aimed to investigate the minimum inhibitory concentration (MIC) and biological properties of modified glass-ionomer cement (BIO-GIC) supplemented with chlorhexidine diacetate (CHX).Methods: The BIO-GIC was originally a combining formulation of conventional glass-ionomer cement, chitosan, albumin and tricalcium phosphate. CHX was incorporated at various concentrations of 1.25%, 2%, 2.5%, 5%, 7.5% and 10%. Antimicrobial activity against certain microorganisms related to cariogenesis Streptococcus mutans (S. mutans), Streptococcus sanguinis (S. sanguinis), and Enterococcus faecalis (E. faecalis), was evaluated by microdilution test. One effective concentration of CHX was mixed with Penaeus merguiensis-Translationally Controlled Tumor Protein (Pmer-TCTP) and further conducted by biological tests including cell viability and mineralization in dental pulp.Results: S. mutans were inhibited at 7.5% CHX-modified BIO-GIC, while S. sanguinis and E. faecalis showed no inhibition at any concentration. Cell viability significantly decreased with 7.5% CHX-modified BIO-GIC after 72 h. Dental pulp exposed to this group formed smaller and fewer calcium nodules but adding Pmer-TCTP reduced cell death and slightly enhanced mineralization.Conclusion: 7.5% CHX-modified BIO-GIC shows potential for inhibiting S. mutans and having potential cell viability and mineralization in dental pulp cells.
Funding Information
  • National Science, Research and Innovation Fund
  • Prince of Songkla University (DEN6801013S)

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