Binding of general transcription factor TFIIB to an acidic activating region
Authors:
- Ilho HaIHIlho Ha
- Edio MaldonadoEMEdio Maldonado
- Danny ReinbergDRDanny Reinberg
- Michael R. GreenMGMichael R. Green
- 1 October 1991
- journal article
- Published by Springer Nature in Nature
- Vol. 353 (6344) , 569-571
Abstract
A central issue in eukaryotic transcriptional regulation is the mechanism by which promoter-specific transcription factors (activators) stimulate transcription. Two lines of evidence indicate that the general transcription factor TFIIB is a pivotal component in the mechanism by which an acidic activator functions. First, during assembly of the preinitiation complex TFIIB binding is a rate-limiting step enhanced by an acidic activator. Second, the TFIIB activity in a HeLa cell nuclear extract is specifically retained on a column containing an acidic activating region. But because our previous study monitored only TFIIB activity, it remains possible that the interaction between TFIIB and the acidic activating region is mediated through additional proteins, for example, those designated as adaptors, coactivators or mediators. A complementary clone encoding TFIIB has recently been isolated and shown to encode a polypeptide of relative molecular mass 35,000. Here we report that TFIIB expressed in and purified from Escherichia coli (recombinant TFIIB) binds directly to the potent acidic activating region of the herpes simplex virus-1 VP16 protein.Keywords
This publication has 19 references indexed in Scilit:
- Reduced binding of TFIID to transcriptionally compromised mutants of VP16Nature, 1991
- A mediator required for activation of RNA polymerase II transcription in vitroNature, 1991
- Mechanism of action of an acidic transcriptional activator in vitroCell, 1991
- Cloning of a Transcriptionally Active Human TATA Binding FactorScience, 1990
- Mechanism of transcriptional activation by Sp1: Evidence for coactivatorsCell, 1990
- A novel mediator between activator proteins and the RNA polymerase II transcription apparatusCell, 1990
- Cloning and structure of a yeast gene encoding a general transcription initiation factor TFIID that binds to the TATA boxNature, 1989
- How eukaryotic transcriptional activators workNature, 1988
- GAL4 derivatives function alone and synergistically with mammalian activators in vitroCell, 1988
- Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferaseGene, 1988