Depolarisation of the plasma membrane in the arsenic trioxide (As2O3)‐and anti‐CD95‐induced apoptosis in myeloid cells
- 17 November 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 578 (1-2) , 85-89
Abstract
Depolarisation of the plasma membrane has been shown to be actively regulated during lymphocyte‐apoptosis. Here, we present data about anti‐Fas and As2O3 induced depolarisation of myeloid U‐937 cells. Anti‐Fas but not As2O3‐induced depolarisation was significantly dependent on caspase‐activation. Na+‐fluxes contributed to the depolarisation in early stages of As2O3‐induced apoptosis, whereas the membrane potential in late stages depended on Cl−‐fluxes. Cl−‐channels also played an important role in the induction of cell shrinkage in As2O3‐induced apoptosis. However, none of these ions contributed significantly to anti‐Fas induced depolarisation. This indicates the existence of different mechanisms for apoptotic plasma membrane depolarisation within one cell type.Keywords
This publication has 19 references indexed in Scilit:
- All-trans retinoic acid/As 2 O 3 combination yields a high quality remission and survival in newly diagnosed acute promyelocytic leukemiaProceedings of the National Academy of Sciences, 2004
- Depolarization of Mitochondria and Activation of Caspases Are Common Features of Arsenic(III)-Induced Apoptosis in Myelogenic and Lymphatic Cell LinesChemical Research in Toxicology, 2004
- The role of mitochondrial targeting in arsenic trioxide‐induced apoptosis in myeloid cell linesBritish Journal of Haematology, 2003
- Uncoupling Cell Shrinkage from Apoptosis Reveals That Na+ Influx Is Required for Volume Loss during Programmed Cell DeathJournal of Biological Chemistry, 2003
- The K+ channel openers diazoxide and NS1619 induce depolarization of mitochondria and have differential effects on cell Ca2+ in CD34+ cell line KG-1aExperimental Hematology, 2003
- Plasma Membrane Depolarization without Repolarization Is an Early Molecular Event in Anti-Fas-induced ApoptosisJournal of Biological Chemistry, 2001
- Normotonic cell shrinkage because of disordered volume regulation is an early prerequisite to apoptosisProceedings of the National Academy of Sciences, 2000
- Complete Remission after Treatment of Acute Promyelocytic Leukemia with Arsenic TrioxideNew England Journal of Medicine, 1998
- Potent block of volume‐activated chloride currents in endothelial cells by the uncharged form of quinine and quinidineBritish Journal of Pharmacology, 1996
- Chloride and potassium channels in U937 human monocytesThe Journal of Membrane Biology, 1990