High throughput production of mouse monoclonal antibodies using antigen microarrays
- 27 October 2005
- journal article
- research article
- Published by Wiley in Proteomics
- Vol. 5 (16) , 4070-4081
Abstract
Recent advances in proteomics research underscore the increasing need for high‐affinity monoclonal antibodies, which are still generated with lengthy, low‐throughput antibody production techniques. Here we present a semi‐automated, high‐throughput method of hybridoma generation and identification. Monoclonal antibodies were raised to different targets in single batch runs of 6–10 wk using multiplexed immunisations, automated fusion and cell‐culture, and a novel antigen‐coated microarray‐screening assay. In a large‐scale experiment, where eight mice were immunized with ten antigens each, we generated monoclonal antibodies against 68 of the targets (85%), within 6 wk of the primary immunization.Keywords
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