An estrogen receptor alpha activity indicator model in mice
Abstract
Estrogen receptor alpha (ERα), plays essential roles in the female reproduction. To investigate the dynamic changes in ERα activity in vivo, we have developed an ER Alpha Activity Indicator (ERAAI) mouse. This ERAAI mouse harbors both a modified ERα Bacterial Artificial Chromosome (BAC) clone and a reporter gene which is regulated specifically by the modified receptor. The ERα modification (Gal4‐ERα) consists replacing the DNA binding domain (DBD) of ERα with the DBD of yeast Gal4 transcription factor. This reporter transgene consisting of a humanized renilla Green Fluorescent Protein (hrGFP) sequence controlled by the Upstream Activating Sequences for the Gal4 gene (UASG) was inserted into the modified ERα BAC clone. Expression of Gal4‐ERα and hrGFP reliably recapitulates endogenous ERα expression and activity in the estrogen target tissues in response to estrogen stimulation. Therefore, the ERAAI mouse represents a novel animal model to investigate dynamic ERα activity in vivo. genesis 47:815–824, 2009.Keywords
This publication has 27 references indexed in Scilit:
- Estrogen Receptor β: An Overview and UpdateNuclear Receptor Signaling, 2008
- The dynamics of estrogen receptor activityMaturitas, 2006
- Emerging Selective Estrogen Receptor ModulatorsDrugs, 2006
- Dynamic Cell Type Specificity of SRC-1 Coactivator in Modulating Uterine Progesterone Receptor Function in MiceMolecular and Cellular Biology, 2005
- A gene expression atlas of the central nervous system based on bacterial artificial chromosomesNature, 2003
- Highly Efficient Modification of Bacterial Artificial Chromosomes (BACs) Using Novel Shuttle Vectors Containing the R6Kγ Origin of ReplicationGenome Research, 2002
- Estrogen receptors and endocrine diseases: lessons from estrogen receptor knockout miceCurrent Opinion in Pharmacology, 2001
- A Highly Efficient Escherichia coli-Based Chromosome Engineering System Adapted for Recombinogenic Targeting and Subcloning of BAC DNAGenomics, 2001
- Alteration of reproductive function but not prenatal sexual development after insertional disruption of the mouse estrogen receptor gene.Proceedings of the National Academy of Sciences, 1993
- Estrogen Receptor in the Rabbit Corpus LuteumScience, 1971