Separation, pre‐concentration, and HPLC analysis of methylxanthines in urine samples
Authors:
- Irena BaranowskaIBIrena Baranowska
- 6 October 2004
- journal article
- research article
- Published by Wiley in Journal of Separation Science
- Vol. 27 (14) , 1166-1172
Abstract
An SPE method, using RP18 phases, for the simultaneous extraction of caffeine, theobromine, theophylline, paraxanthine, 1‐methylxanthine, 3‐methylxanthine, 7‐methylxanthine, 1‐methyluric acid, 1,3‐dimethyluric acid, 1,7‐dimethyluric acid, and 1,3,7‐trimethyluric acid from urine has been developed. Besides a gradient HPLC system for the analysis of the compounds of interest on a LiChrosorb RP‐18 (7 μm) column with mobile phase containing 0.05% aq. solution of trifluoroacetic acid and acetonitrile has been elaborated. The procedure has been successfully applied to the analysis of methylxanthines and methyluric acids in urine of patients with chronic asthma treated with theophylline and in urine of healthy subjects.Keywords
This publication has 18 references indexed in Scilit:
- Caffeine and nutritionNutrition, 2000
- Extraction of methylxanthines from human body fluids by solid-phase microextractionAnalytica Chimica Acta, 1999
- SIMULTANEOUS DETERMINATION OF METHYLURIC ACIDS IN BIOLOGICAL FLUIDS BY RP-HPLC ANALYSIS AFTER SOLID PHASE EXTRACTIONJournal of Liquid Chromatography & Related Technologies, 1999
- Threshold level for theophylline in doping analysisJournal of Chromatography B: Biomedical Sciences and Applications, 1996
- Determination of theophylline and its metabolites in human urine and plasma by high-performance liquid chromatographyJournal of Chromatography B: Biomedical Sciences and Applications, 1996
- Simultaneous assay of fluoroquinolones and theophylline in plasma by high-performance liquid chromatographyJournal of Chromatography B: Biomedical Sciences and Applications, 1993
- A Class-Independent Drug Screen in Forensic Toxicology Using a Photodiode Array DetectorJournal of Analytical Toxicology, 1993
- Simultaneous determination of caffeine and its primary demethylated metabolites in human plasma by high-performance liquid chromatographyJournal of Chromatography B: Biomedical Sciences and Applications, 1992
- Developmental Changes of Caffeine Elimination in InfancyDevelopmental Pharmacology and Therapeutics, 1988
- Methylxanthines: Toxicity to humans. 1. TheophyllineFood and Chemical Toxicology, 1988