Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation
Authors:
- James D HirschJHJames D Hirsch
- Leila EslamizarLELeila Eslamizar
- Brian J FilanoskiBFBrian J Filanoski
- Nabi MalekzadehNMNabi Malekzadeh
- Joseph M BeechemJBJoseph M Beechem
- Richard P HauglandRHRichard P Haugland
- 1 September 2002
- journal article
- Published by Elsevier in Analytical Biochemistry
- Vol. 308 (2) , 343-357
Abstract
No abstract availableKeywords
This publication has 39 references indexed in Scilit:
- EditorialBiomolecular Engineering, 1999
- A streptavidin mutant with altered ligand-binding specificityProceedings of the National Academy of Sciences, 1998
- Identification of Biotinylation Sites on Proteins by Selective Retrieval of 2-Iminobiotinylated Peptides from Proteolytic Peptide Mixtures: Localization of the Accessible Lysine Residues on the Photosystem I Subunits PsaD and PsaEAnalytical Biochemistry, 1998
- Recovery of High-Affinity Phage from a Nitrostreptavidin Matrix in Phage-Display TechnologyAnalytical Biochemistry, 1996
- Immobilized Nitro-avidin and Nitro-streptavidin as Reusable Affinity Matrices for Application in Avidin–Biotin TechnologyAnalytical Biochemistry, 1996
- Reversibility of biotin-binding by selective modification of tyrosine in avidinBiochemical Journal, 1996
- Assessment of a positive selection technique using an avidin column to isolate human peripheral blood T cell subsetsJournal of Immunological Methods, 1994
- Elimination of Daudi lymphoblasts from human bone marrow using avidin- biotin immunoadsorptionBlood, 1986
- An analytical method for the selective retrieval of iminobiotin-derivatized plasma membrane proteinsAnalytical Biochemistry, 1983
- The properties of subunits of avidin coupled to SepharoseBiochemical Journal, 1973