Th-1, Th-2 Cytokines Profile among Madurella mycetomatis Eumycetoma Patients

Authors:
Open Access
Publisher website
Google Scholar
Abstract
Eumycetoma is a progressive and destructive chronic granulomatous subcutaneous inflammatory disease caused by certain fungi, the most common being Madurella mycetomatis. The host defence mechanisms against fungi usually range from an early non-specific immune response to activation and induction of specific adaptive immune responses by the production of Th-1 and Th-2 cytokines. The aim of this study is to determine the levels of Th-1 and Th-2 cytokines in patients infected with Madurella mycetomatis, and the association between their levels and disease prognosis. This is a descriptive cross-sectional study conducted at the Mycetoma Research Centre, University of Khartoum, Sudan, where 70 patients with confirmed M. mycetomatis eumycetoma were enrolled; 35 with, and 35 without surgical excision. 70 healthy individuals from mycetoma endemic areas were selected as controls. The levels of serum cytokines were determined by cytometric bead array technique. Significantly higher levels of the Th-1 cytokines (IFN-γ, TNF-α, IL-1β and IL-2) were recorded in patients treated with surgical excision, compared to those treated without surgical excision. In contrast, the Th-2 cytokines (IL-4, IL-5, IL-6 and IL-10) were significantly lower in patients treated with surgical excision compared to those treated without surgical excision. In conclusion, the results of this study suggest that cell-mediated immunity can have a role to play in the pathogenesis of eumycetoma. Madurella mycetomatis is the most common causative agent for eumycetoma, which is a progressive and destructive subcutaneous inflammatory disease. It is a neglected tropical disease affecting the population in poor and remote endemic tropical and subtropical areas. Currently, the susceptibility and resistance to mycetoma are not well defined, and many factors can be incriminated, including immunological, genetic, or environmental ones. The current descriptive cross-sectional study was conducted to determine the Th-1 and Th-2 cytokine levels among 70 patients with Madurella mycetomatis eumycetoma and 70 healthy controls. It aimed to find out the association between the disease prognosis and the level of these cytokines. Significantly higher levels of the Th-1 cytokines (IFN-γ, TNF-α, IL-1β and IL-2) were found in patients treated with surgical excision compared to those treated without surgical intervention. However, the Th-2 cytokines (IL-4, IL-5, IL-6 and IL-10) were significantly lower in patients treated with surgical excision compared to those treated without surgical excision. These findings suggested that, cell-mediated immunity has a prime role in the pathogenesis of eumycetoma.
Funding Information
  • King Abdullah International Medical Research Centre (KAIMRC), National Guard Health Affairs, Kingdom of Saudi Arabia

This publication has 35 references indexed in Scilit: