Unveiling Embryonic Development of the Threatened Neotropical Fish Prochilodus vimboides (Characiformes: Prochilodontidae)

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Abstract
Understanding embryonic development is fundamental to improving captive breeding protocols and supporting conservation strategies for threatened fish species. Prochilodus vimboides is a Neotropical freshwater fish for which detailed information on early ontogeny remains scarce. This study aimed to characterize the embryonic and early larval development of P. vimboides under captive conditions. Broodstock were hormonally induced to reproduce, and extrusion occurred between 209 and 230 degree-hours after induction at 21.49 ± 0.15 °C. Embryonic development was monitored at regular intervals after fertilization (AF) using freshly collected eggs examined under a stereomicroscope. The principal developmental stages were identified, namely zygote, cleavage, including morula and blastula, gastrula, organogenesis, and hatching. Fertilized oocytes exhibited marked hydration and formation of a large perivitelline space at 15 min AF. More than 50% of embryos reached the two-blastomere stage by 20 min AF, and cleavage continued until 2 h 14 min AF. The gastrula stage was observed at 3 h 23 min AF, blastopore closure occurred at 11 h 47 min AF, and organogenesis began at 12 h 55 min AF. Complete hatching occurred at 22 h 04 min AF, and larvae subsequently initiated yolk sac absorption without cannibalistic behavior. These findings provide a species-specific developmental framework that supports captive production and conservation efforts for P. vimboides in the Paraíba do Sul River Basin.
Funding Information
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP 2014/16320-7)
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (2017/06765-0)
  • CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) productivity scholarship
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Finance Code 001)

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