Physical interactions among circadian clock proteins KaiA, KaiB and KaiC in cyanobacteria
Authors:
Open Access
- 1 March 1999
- journal article
- research article
- Published by Springer Nature in The EMBO Journal
- Vol. 18 (5) , 1137-1145
Abstract
The kai gene cluster, which is composed of three genes, kaiA, kaiB and kaiC , is essential for the generation of circadian rhythms in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942. Here we demonstrate the direct association of KaiA, KaiB and KaiC in yeast cells using the two‐hybrid system, in vitro and in cyanobacterial cells. KaiC enhanced KaiA–KaiB interaction in vitro and in yeast cells, suggesting that the three Kai proteins were able to form a heteromultimeric complex. We also found that a long period mutation kaiA1 dramatically enhanced KaiA–KaiB interaction in vitro . Thus, direct protein–protein association among the Kai proteins may be a critical process in the generation of circadian rhythms in cyanobacteria.Keywords
This publication has 35 references indexed in Scilit:
- Mammalian Circadian Autoregulatory LoopNeuron, 1998
- Role of the CLOCK Protein in the Mammalian Circadian MechanismScience, 1998
- Regulation of Nuclear Entry of the Drosophila Clock Proteins Period and TimelessNeuron, 1996
- A light-entrainment mechanism for the Drosophila circadian clockNature, 1996
- Temporally regulated nuclear entry of the Drosophila period protein contributes to the circadian clockNeuron, 1995
- Circadian Clock Mutants of CyanobacteriaScience, 1994
- Feedback of the Drosophila period gene product on circadian cycling of its messenger RNA levelsNature, 1990
- A novel genetic system to detect protein–protein interactionsNature, 1989
- Product of per locus of Drosophila shares homology with proteoglycansNature, 1986
- Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomesCell, 1986