An improved one‐pot preparation of [18F]FMISO based on solid phase extraction purification: Pitfalls on the analytical method reported in the Ph.Eur.'s monograph
Authors:
- 6 October 2021
- journal article
- research article
- Published by Wiley in Journal of Labelled Compounds and Radiopharmaceuticals
- Vol. 65 (1) , 6-12
Abstract
By adapting previously reported reaction conditions, [18F]FMISO was synthesized using a TracerLab FX‐FDG module in 31 min from no carrier added [18F]fluoride in 56% radiochemical yield. A novel and simple purification setup based on disposable cartridges was developed allowing the radiopharmaceutical to be obtained in high radiochemical purity (>99.5%) and with chemical impurities far below the Ph.Eur.'s thresholds. Moreover, the study pinpointed some shortcomings of the high‐performance liquid chromatography (HPLC) method reported in the dedicated Ph.Eur's monograph.Keywords
This publication has 16 references indexed in Scilit:
- Automated synthesis and PET evaluation of both enantiomers of [18F]FMISONuclear Medicine and Biology, 2015
- Development of a new precursor‐minimizing base control method and its application for the automated synthesis and SPE purification of [18F]fluoromisonidazole ([18F]FMISO)Journal of Labelled Compounds and Radiopharmaceuticals, 2013
- The possibility of a fully automated procedure for radiosynthesis of fluorine-18-labeled fluoromisonidazole using a simplified single, neutral alumina column purification procedureApplied Radiation and Isotopes, 2010
- Automated synthesis of hypoxia imaging agent [18F]FMISO based upon a modified Explora FDG4 moduleJournal of Radioanalytical and Nuclear Chemistry, 2009
- The Presence of Ethanol in Radiopharmaceutical InjectionsJournal of Nuclear Medicine, 2008
- A robotic synthesis of [18F]fluoromisonidazole ([18F]FMISO)Applied Radiation and Isotopes, 2007
- Fully automated synthesis of [18F]fluoromisonidazole using a conventional [18F]FDG moduleNuclear Medicine and Biology, 2005
- Fully automated one-pot synthesis of [18F]fluoromisonidazoleNuclear Medicine and Biology, 2005
- Preparation of fluorine‐18‐labelled fluoromisonidazole using two different synthesis methodsJournal of Labelled Compounds and Radiopharmaceuticals, 2003
- Synthesis and biodistribution of 18F-labeled fluoronitroimidazoles: Potential in vivo markers of hypoxic tissueApplied Radiation and Isotopes, 1986