Responses of GPCR135 to Human Gene 3 (H3) Relaxin in CHO‐K1 Cells Determined by Microphysiometry
- 1 May 2005
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 1041 (1) , 332-337
Abstract
Abstract: This study examined the functional response to human relaxin 2 (H2 relaxin), human relaxin 3 (H3 relaxin), porcine relaxin, and human INSL3 in the cytosensor microphysiometer, using CHO‐K1 cells stably expressing human GPCR135. CHO‐K1 cells stably expressing GPCR135 were generated by the serial dilution method and receptor properties were assessed. Saturation studies of [125I] H3 relaxin binding to GPCR135 in these cells gave a Bmax of 32.61 ± 6.5 fmol/mg protein and Kd of 0.12 ± 0.08 nM. The functional response to H3 relaxin and other relaxin/insulin peptides of GPCR135 expressed in CHO‐K1 cells was measured in the cytosensor microphysiometer and analyzed using inhibitors of signal transduction proteins.Keywords
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