A novel nucleic acid linker for multi‐gene expression enhances plant and animal synthetic biology

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Abstract
SUMMARY: The production of compact vectors for gene stacking is hindered by a lack of effective linkers. Here, we report that a 26‐nt nucleic acid linker, NAL1, from the fungus Glarea lozoyensis and its truncated derivatives could connect two genes as a bicistron, enabling independent translation in a maize protoplast transient expression system and human 293 T cells. The optimized 9‐nt NAL10 linker was then used to connect four genes driven by a bidirectional promoter; this combination was successfully used to reconstruct the astaxanthin biosynthesis pathway in transgenic maize. The short and efficient nucleic acid linker NAL10 can be widely used in multi‐gene expression and synthetic biology in animals and plants.
Funding Information
  • National Key Research and Development Program of China (2023ZD0403005)
  • National Natural Science Foundation of China (32171453, 32272118)
  • Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences (1610392020001)

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