Nucleic Acid-binding Properties of Adenovirus Structural Polypeptides
Authors:
Open Access
- 1 November 1982
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 63 (1) , 69-79
Abstract
The ability of adenovirus structural polypeptides to bind nucleic acids was assessed by separating the polypeptides on SDS-polyacrylamide gels, transferring them electrophoretically to nitrocellulose and probing with 32P-labelled nucleic acids. Polypeptides IVa2, V, VI and VII, as well as trace amounts of pVII and a polypeptide of apparent mol. wt. 40 X 10(3) were able to bind label under these conditions. Labelling was also detected with a smaller polypeptide, possibly related to the cleavage products of pVII and/or pVI. The binding of DNA to polypeptide VI appeared to be more sensitive to detergents than the others. No sequence specificity could be detected in the DNA binding.Keywords
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