2D and 3D in vitro Assays to Quantify the Invasive Behavior of Glioblastoma Stem Cells in Response to SDF-1α
Open Access
- 1 September 2020
- journal article
- research article
- Published by Taylor & Francis in BioTechniques
- Vol. 69 (5) , 339-346
Abstract
Invasion is a hallmark of cancer and therefore in vitro invasion assays are important tools in cancer research. We aimed to describe in vitro 2D transwell assays and 3D spheroid assays to quantitat... METHOD SUMMARY We describe 2D transwell invasion assays and 3D spheroid invasion assays for the investigation of effects of the chemoattractant SDF-1α on human glioblastoma stem cells in vitro in a...Keywords
Funding Information
- European Program of Cross-Border Cooperation for Slovenia-Italy Interreg TRANS-GLIOMA
- IVY Interreg Fellowship 2018
- Dutch Cancer Society (UVA 2014-6839 and UVA 2016-10460)
- Slovenian Research Agency (Program P10245)
- The Fondation pour la Recherche Nuovo-Soldati 2019
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