Activity of human, bovine and porcine platelet‐derived growth factor in a radioreceptor assay with human placental membrane protein

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Abstract
Radioiodinated human platelet‐derived growth factor (125I‐PDGF), and unlabeled human (Hu), bovine (Bo), and porcine (Po) PDGF were used to study PDGF receptors in human term placental membrane preparations. The binding of 125I‐Hu‐PDGF was inhibited by unlabeled preparations. Half‐maximal occupancy of the binding sites occurred at 7–9 nM of Hu‐, Bo‐ or Po‐PDGF. Scatchard analysis of the binding data indicated a single class of receptors having a K d value of about 1.1–1.2 × 10−10 M. Thus, the sensitive radioreceptor assay does not detect any species specificity of PDGF.

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