Effects of dietary resistant starch content on nutrient and energy digestibility and fecal metabolomic profile in growing pigs1

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Abstract
Ten ileal-cannulated barrows (65.1 ± 2.8 kg initial BW) were used to determine the effects of dietary resistant starch (RS) content on apparent ileal digestibility (AID), apparent total tract digestibility (ATTD), and hindgut fermentation (HGF) of DM, GE, N, Ca, and P, as well as on fecal metabolomic profile. Pigs were allotted to a randomized complete block design with 2 diets containing low- or high-RS contents and 5 replicate pigs per diet. After a 7-d adaptation period, ileal digesta and feces were collected for 4 and 3 d, respectively. Chromic oxide was included (0.5%) in the diets as an indigestible marker. Nontargeted metabolomics analysis of fecal water samples was performed using mass spectrometry. The AID of nutrient and energy were not affected by the treatments, whereas ATTD of DM, GE, and Ca decreased (P < 0.05) in pigs fed the high-RS diet. Feeding the high-RS diet to pigs increased (P = 0.03) HGF of DM, tended (P = 0.06) to increase HGF of GE, and decreased (P = 0.04) HGF of N. Ninety-seven metabolites, including AA and lipids, differed (P < 0.01) between low- and high-RS diets. Principal component analysis revealed 2 distinct clusters of metabolite profiles between treatments. Results show that RS-rich diets decrease total tract nutrient and energy digestibility with a concomitant increase in HGF of DM and GE and that fecal water is a suitable biofluid for biomarker discovery in metabolomics studies in pigs.
Funding Information
  • Ingredion Inc.

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