From people to Panthera : Natural SARS-CoV-2 infection in tigers and lions at the Bronx Zoo
Authors:
- Denise McAlooseDMDenise McAloose
- Melissa LaverackMLMelissa Laverack
- Leyi WangLeyi Wang
- Mary Lea KillianMKMary Lea Killian
- Leonardo C. CasertaLCLeonardo C. Caserta
- Fangfeng YuanFYFangfeng Yuan
- Patrick K. MitchellPMPatrick K. Mitchell
- Krista QueenKQKrista Queen
- Brittany D. CronkBCBrittany D. Cronk
- Susan L. BartlettSBSusan L. Bartlett
- John M. SykesJSJohn M. Sykes
Preprint
Abstract
We describe the first cases of natural SARS-CoV-2 infection detected in animals in the United States. In March 2020, four tigers and three lions at the Bronx Zoo developed mild respiratory signs. SARS-CoV-2 RNA was detected by rRT-PCR in respiratory secretions and/or feces from all seven affected animals; viral RNA and/or antibodies were detected in their keepers. SARS-CoV-2 was isolated from respiratory secretions or feces from three affected animals; in situ hybridization co-localized viral RNA with cellular damage. Whole genome sequence and haplotype network analyses showed tigers and lions were infected with two different SARS-CoV-2 strains, suggesting independent viral introductions. The source of SARS-CoV-2 infection in the lions is unknown. Epidemiological data and genetic similarities between keeper and tiger viruses indicate human to animal transmission.Keywords
All Related Versions
- Published version: mBio, 11 (5).
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