Genome-wide DNA methylation profiling of non-small cell lung carcinomas
Authors:
- Rejane Hughes CarvalhoRCRejane Hughes Carvalho
- Vanja HaberleVHVanja Haberle
- Jun HouJHJun Hou
- Teus van GentTGTeus van Gent
- Supat ThongjueaSTSupat Thongjuea
- Wilfred van IjckenWIWilfred van Ijcken
- Christel KockxCKChristel Kockx
- Rutger BrouwerRBRutger Brouwer
- Erikjan RijkersERErikjan Rijkers
- Anieta SieuwertsASAnieta Sieuwerts
- John FoekensJFJohn Foekens
- Mirjam van VroonhovenMVMirjam van Vroonhoven
Open Access
- 22 June 2012
- journal article
- research article
- Published by Springer Nature in Epigenetics & Chromatin
- Vol. 5 (1) , 9
Abstract
Non-small cell lung carcinoma (NSCLC) is a complex malignancy that owing to its heterogeneity and poor prognosis poses many challenges to diagnosis, prognosis and patient treatment. DNA methylation is an important mechanism of epigenetic regulation involved in normal development and cancer. It is a very stable and specific modification and therefore in principle a very suitable marker for epigenetic phenotyping of tumors. Here we present a genome-wide DNA methylation analysis of NSCLC samples and paired lung tissues, where we combine MethylCap and next generation sequencing (MethylCap-seq) to provide comprehensive DNA methylation maps of the tumor and paired lung samples. The MethylCap-seq data were validated by bisulfite sequencing and methyl-specific polymerase chain reaction of selected regions.Keywords
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