Covalent Attachment of Methylene Blue to Oligonucleotides
Authors:
- 1 August 1995
- journal article
- research article
- Published by Taylor & Francis in Nucleosides and Nucleotides
- Vol. 14 (6) , 1437-1443
Abstract
The synthesis and application of oligonucleotides derivatized by methylene blue are described. For that, a carboxylated methylene blue derivative was synthesized and transformed into an activated N-hydroxysuccinimidoester. The activated ester was reacted with 5′-aminoalkylated oligonucleotides. The labelled oligonucleotides were isolated and characterized both by reversed phase HPLC and MALDI mass spectrometry. Initial studies on analytical application of these oligonucleotide conjugates are discussed.Keywords
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