ARE THERE LIVING BACTERIA IN PERMANENTLY FROZEN SUBSOIL?

Authors:
  • NJNorman James
    Norman James
  • MSMarjorie L. Sutherland
    Marjorie L. Sutherland
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Abstract
Samples of frozen subsoil were obtained aseptically at Churchill, Man., located on Hudson Bay at about 58° north latitude. These were cultured on various media. The procedure followed failed to demonstrate the presence of viable micro-organisms in the permanently frozen zone of a clay subsoil. Bacteria were isolated from samples obtained as deep as nine feet from the surface of a coarse gravel subsoil.