Generation of an internal matrix in mature avian erythrocyte nuclei during reactivation in cytoplasts.
Authors:
- R E LaFondRLR E LaFond
- H WoodcockHWH Woodcock
- C L WoodcockCWC L Woodcock
- E R KundahlEKE R Kundahl
- J J LucasJLJ J Lucas
Open Access
- 1 June 1983
- journal article
- Published by Rockefeller University Press in Journal of Cell Biology
- Vol. 96 (6) , 1815-1819
Abstract
When fused with mouse L-cell cytoplasts, chick erythrocyte nuclei enlarge, take up proteins from the host cytoplasm, and recommence RNA synthesis. We found that during this transition the erythrocyte nuclei gain an internal nuclear matrix, thus providing a novel approach to questions concerning the nature of the salt-resistant intranuclear skeleton. A new method for preparation and examination of the nuclear matrix in situ is also described.Keywords
This publication has 41 references indexed in Scilit:
- Association of globin RNA and its precursors with the chicken erythroblast nuclear matrixBiochemistry, 1982
- The nuclear matrix of duck erythroblasts is associated with globin mRNA coding sequences but not with the major proteins of 40S nuclear RNPExperimental Cell Research, 1981
- RNA is synthesized at the nuclear cageNature, 1981
- Considerations in the isolation of rat liver nuclear matrix, nuclear envelope, and pore complex laminaExperimental Cell Research, 1981
- Sequence analysis of nuclear matrix associated DNA from rat liverExperimental Cell Research, 1980
- Functional differences between mammalian nuclear protein matrices and pore-lamina complex laminaeExperimental Cell Research, 1979
- Effect of protease inhibitors on matrix proteins and the association of replicating DNAExperimental Cell Research, 1979
- Isolation and characterization of an RNA-containing nuclear matrix from Tetrahymena macronucleiBiochemistry, 1979
- Cell cycle dependence of the reactivation of chick erythrocyte nuclei after transplantation into mouse L929 cell cytoplastsJournal of Cellular Physiology, 1978
- Isolation of a nuclear ribonucleoprotein network that contains heterogeneous RNA and is bound to the nuclear envelopeBiochemistry, 1975