The Inhibition Effect of Cold Atmospheric Plasma-Activated Media in Cutaneous Squamous Carcinoma Cells

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Abstract
Aim: This study investigated the effect and mechanism of cold atmospheric plasma (CAP)-activated media on A431 and HaCaT cells. Materials & methods: Phosphate-buffered saline (PBS) and Dulbecco’s Modified Eagle's Medium (DMEM) were treated by CAP to get CAP-activated media. A431 and HaCaT were incubated by CAP-activated media for 2 h. MTT, Annexin-V and propidium iodide (PI), Western blot and reactive oxygen species (ROS) detection assay were utilized to demonstrate the effect. Results: The CAP-activated media decreased the proliferation of A431 cells in a dose/time-dependent manner. And the CAP-activated media could induce apoptosis in A431 cells. CAP-activated phosphate-buffered saline could increase intracellular ROS level but not CAP-activated DMEM. Conclusion: CAP-activated media could induce apoptosis in A431 cells by production of ROS.
Funding Information
  • foundation of the Second Affiliated Hospital of Anhui Medical University (2015hhjh04)
  • National Natural Science Foundation of China (51777206, 31600680, 81673099, 31670860, 81602083, 31800702)
  • Anhui Provincial Natural Science Foundation (1608085QH216, 1708085MA13)
  • Grants for Scientific Research of BSKY (XJ201505)

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