CRYOPRESERVATION OF HUMAN CADAVER CORNEAS REGENERATED AT 31°N A MODIFIED TISSUE CULTURE MEDIUM
Author:
- 31 January 1981
- journal article
- research article
- Published by Wiley in Acta Ophthalmologica
- Vol. 59 (1) , 142-148
Abstract
Human corneas were obtained 2-82 h post mortem, cultured for 20-28 h in a modified tissue culture medium, frozen at a controlled rate and thawed rapidly. The thawed corneas were subjected to 20-28 h of additional culture. Immediately after thawing, a mean endothelial cell damage of 11% was indicated by trypane blue staining. The mean endothelial cell loss during the subsequent culture was 34%. This cell loss was not related to post mortem time, to donor age, to cell loss during the primary culture or to endothelial cell density.Keywords
This publication has 9 references indexed in Scilit:
- FRESH AND CULTURED CORNEAL GRAFTS COMPARED BY POST‐OPERATIVE THICKNESS AND ENDOTHELIAL CELL DENSITYActa Ophthalmologica, 1981
- A SIMPLE APPARATUS FOR CONTROLLED RATE CORNEAL FREEZING IIActa Ophthalmologica, 1981
- DECONTAMINATION OF CADAVER CORNEASActa Ophthalmologica, 1981
- TOXICITY OF DIMETHYLSULFOXIDE (DMSO) TO HUMAN CORNEAL ENDOTHELIUM IN VITROActa Ophthalmologica, 1979
- EARLY MORPHOLOGICAL CHANGES IN ORGAN CULTURED HUMAN CORNEAL ENDOTHELIUMActa Ophthalmologica, 1978
- THE PRECISION OF UNBIASED ESTIMATES OF NUMERICAL DENSITY OF ENDOTHELIAL CELLS IN DONOR CORNEASActa Ophthalmologica, 1978
- COMBINED STAINING OF CORNEAL ENDOTHELIUM BY ALIZARINE RED AND TRYPANE BLUEActa Ophthalmologica, 1977
- Full-thickness corneal grafts in Addis Ababa, Ethiopia.British Journal of Ophthalmology, 1967
- Preservation of Viable Corneal TissueArchives of Ophthalmology (1950), 1965