KaiB functions as an attenuator of KaiC phosphorylation in the cyanobacterial circadian clock system
Open Access
- 1 May 2003
- journal article
- research article
- Published by Springer Nature in The EMBO Journal
- Vol. 22 (9) , 2127-2134
Abstract
In the cyanobacterium Synechococcus elongatus PCC 7942, the KaiA, KaiB and KaiC proteins are essential for generation of circadian rhythms. We quantitatively analyzed the intracellular dynamics of these proteins and found a circadian rhythm in the membrane/cytosolic localization of KaiB, such that KaiB interacts with a KaiA–KaiC complex during the late subjective night. KaiB–KaiC binding is accompanied by a dramatic reduction in KaiC phosphorylation and followed by dissociation of the clock protein complex(es). KaiB attenuated KaiA‐enhanced phosphorylation both in vitro and in vivo . Based on these results, we propose a novel role for KaiB in a regulatory link among subcellular localization, protein–protein interactions and post‐translational modification of Kai proteins in the cyanobacterial clock system.Keywords
This publication has 24 references indexed in Scilit:
- Circadian Formation of Clock Protein Complexes by KaiA, KaiB, KaiC, and SasA in CyanobacteriaJournal of Biological Chemistry, 2003
- Circadian clock protein KaiC forms ATP-dependent hexameric rings and binds DNAProceedings of the National Academy of Sciences, 2002
- Structure and function from the circadian clock protein KaiA of Synechococcus elongatus : A potential clock input mechanismProceedings of the National Academy of Sciences, 2002
- "What watch? ... such much!"* Complexity and evolution of circadian clocksCell and tissue research, 2002
- A KaiC-Interacting Sensory Histidine Kinase, SasA, Necessary to Sustain Robust Circadian Oscillation in CyanobacteriaCell, 2000
- Expression of a Gene Cluster kaiABC as a Circadian Feedback Process in CyanobacteriaScience, 1998
- Cloning of Human PEXcDNAJournal of Biological Chemistry, 1998
- CYANOBACTERIAL CIRCADIAN RHYTHMSAnnual Review of Plant Biology, 1997
- Identification and Characterization of the ictA/ndhL Gene Product Essential to Inorganic Carbon Transport of Synechocystis PCC6803Plant Physiology, 1992
- Translational efficiency of the Escherichia coli adenylate cyclase gene: mutating the UUG initiation codon to GUG or AUG results in increased gene expression.Proceedings of the National Academy of Sciences, 1985