A competitive direct enzyme-linked immunosorbent assay for the rapid detection of deoxynivalenol: development and application in agricultural products and feedstuff

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Abstract
A competitive direct enzyme-linked immunosorbent assay (cd-ELISA) was developed for the rapid detection of deoxynivalenol (DON) in food and feedstuff. Polyclonal antibodies against DON were generated by immunizing rabbits with 3-HS-DON-BSA conjugates. In this assay, the sensitivity (measured as IC50) and limit of detection (measured as IC15) were 0.03 and 0.003 mg kg−1, respectively. A total of eight sample types (barley, wheat, oat, maize, rice, flour, milk and feedstuff) were chosen to evaluate the cd-ELISA assay performance. The sample could be directly detected after extraction and dilution with double-distilled water. The limit of detection in the samples was in the range of 0.15–0.48 mg kg−1. In the end, the spike and recovery results in both cd-ELISA and high-performance liquid chromatography (HPLC) were compared for assay validation. The recovery results ranged between 70% and 100%. A good correlation (R2 = 0.9613) between ELISA and HPLC was obtained.
Funding Information
  • the Ministry of Science and Technology of the People’s Republic of China (2013BAD18B11])
  • International Science and Technology cooperation program of China (2014DFR30350])
  • the Ministry of Science and Technology of Tianjin (14ZCDGNC00098])
  • the Natural Science Foundation of China (31501566])

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