Development and Trait-Based Molecular Characterization of Thermosensitive Genic Male-Sterile Rice (Oryza sativa L.) Lines at Texas A&M AgriLife Research
Open Access
Abstract
This study aimed to develop and genetically characterize thermosensitive genic male-sterility (TGMS) lines for use in hybrid rice (Oryza sativa L.) breeding. Male-sterile F2 to F4 generation lines were screened during the high-temperature summer season, and ratoon crops of selected male-sterile rows were harvested for pure seed. Sixty-six F5 TGMS lines were genotyped using DNA markers controlling 16 traits from the LSU80 QA/QC Rice PlexSeq SNP Panel. Ten TGMS lines with desirable traits that included semidwarf, glabrous, non-aromatic, long-grain, narrow brown leaf spot resistance, and blast resistance genes were selected for further genotypic characterization using markers for low chalkiness (chalk5), wide compatibility (S5-n), cold tolerance (qSCT-11 and qCST-12), and anaerobic germination (AG1 and AG2). TGMS lines TIL21051S and TIL21052S possess favorable alleles for each of the genes evaluated in this study and are desirable parents for two-line hybrid breeding in the southeast United States. TIL21044S, TIL21095S, TIL21060S, and TIL21066S each contain three blast resistance genes and have potential as parental lines. TIL21014S-2, TIL21015S, and TIL21016S-1 include the fgr allele for aroma and can also be used as parental lines for aromatic two-line hybrids.Keywords
Funding Information
- Texas Rice Research Foundation (124214-94410, 114214-94410, 203463-94410)
- Texas A&M AgriLife Research (124214-94410, 114214-94410, 203463-94410)
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