Alkaline phosphatase activity of normal and cystic fibrosis fibroblasts.
Authors:
- D A AitkenDAD A Aitken
- A HoogeveenAHA Hoogeveen
Open Access
- 1 June 1980
- journal article
- research article
- Published by BMJ in Journal of Medical Genetics
- Vol. 17 (3) , 187-190
Abstract
Alkaline phosphatase (ALP) activities were compared in fibroblasts from three cystic fibrosis patients and two normal controls after culturing the cells in normal growth medium and in medium containing Tamm-Horsfall glycoprotein, isoproterenol, and theophylline. No consistent alterations in ALP activities were noted, either between the same cell lines grown under different conditions, or between normal and cystic cell lines. It is concluded that it is not possible to use changes in ALP activity in cultured cells for the prenatal diagnosis of cystic fibrosis.Keywords
This publication has 16 references indexed in Scilit:
- Decreased Adenosine 3′: 5′-Monophosphate Response to Isoproterenol in Cystic Fibrosis LeukocytesPediatric Research, 1978
- Reliable detection of cystic fibrosis in skin-derived fibroblast culturesHuman Genetics, 1978
- Cystic fibrosis: Leakage of lysosomal enzymes and of alkaline phosphatase into the extracellular spaceBiochemical and Biophysical Research Communications, 1977
- Conditions for induction of alkaline phosphatase in cultured human fetal skin fibroblastsExperimental Cell Research, 1977
- Prospects for prenatal diagnosis of cystic fibrosis: Induction of biochemical abnormalities in fibroblasts from patients with cystic fibrosis by a urinary glycoproteinBiochemical and Biophysical Research Communications, 1976
- The use of quantitative cytochemical analyses in rapid prenatal detection and somatic cell genetic studies of metabolic diseasesJournal of Molecular Histology, 1974
- Changes in the alkaline phosphatase activity of serum samples after thawing and after reconstitution from the lyophilized stateClinica Chimica Acta, 1971
- Enzymes in Cultivated Human Fibroblasts derived from Patients with Down's Syndrome (Mongolism)Nature, 1967
- INDUCTION OF ALKALINE PHOSPHATASE BY SUBSTRATES IN ESTABLISHED CULTURES OF CELLS FROM INDIVIDUAL HUMAN DONORSProceedings of the National Academy of Sciences, 1961
- A MUCOPROTEIN DERIVED FROM HUMAN URINE WHICH REACTS WITH INFLUENZA, MUMPS, AND NEWCASTLE DISEASE VIRUSESJournal of Experimental Medicine, 1952