Characterization of succinimide‐type dehydration products of recombinant hirudin variant 1 by electrospray tandem mass spectrometry
Authors:
- 1 December 1993
- journal article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 7 (12) , 1082-1085
Abstract
Two congeners Q 4 and Q 5 inferred in earlier analyses to be cyclic succinimide‐type dehydration product; of recombinant hirudin (variant 1) were structurally fully characterized. After isotopic labeling by ring‐opening with H 2 18 O, the suspected anhydro‐positions Asp 53 and Asp 33 were confirmed by tandem mass spectrometry (MS/MS) sequencing of relevant smaller peplides directly in the enzymatic hydrolysates (V8 protease) using electrospray MS/MS. The chosen strategy proved highly efficient and sensitive.Keywords
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