Mentha rotundifolia (L.) Huds. aqueous extract attenuates H2O2 induced oxidative stress and neurotoxicity

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Abstract
Introduction: Oxidative stress plays a causal role in neurodegenerative diseases. The aim of this study is to evaluate the antioxidant and neuroprotective effects ofMentha rotundifolia(L.) Huds (M. rotundifolia), a widely used Moroccan plant in traditional medicine.Methods: The chemical composition ofM. rotundifoliaaqueous extract was analyzed by liquid chromatography coupled to mass spectrometry (LC-MS). 2,2-diphenyl 1-picrylhydrazyl (DPPH) and 2,2′-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS+) assays were used to assess itsin vitroantioxidant activity. H2O2was utilized to induce oxidative stress and neurotoxicityin vivo. Behavioral changes were evaluated using Open Field, Y-maze and Rotarod tests. Hyperalgesia was assessed using the tail immersion test.Results and discussion: The LC-MS/MS analysis revealed high content of kaempferol glucuronide (85%) at the extract. IC50values of the DPPH and ABTS were 26.47 and 41.21 μg/mL, respectively. Pre-treatments withM. rotundifoliaextract attenuated the behavioral changes induced by H2O2. In addition, the latency of tail withdrawal increased significantly in the treated groups suggesting central analgesic effect ofM. rotundifoliaextract. Moreover, the extract attenuated the deleterious effects of H2O2and improved all liver biomarkers. The obtained results suggested thatM. rotundifoliahad remarkable antioxidant and neuroprotective effects and may prevent oxidative stress related disorders.

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