Complete amino acid sequence of a new type of lethal neurotoxin from the venom of the funnel‐web spider Atrax robustus
Authors:
- D.D. SheumackDSD.D. Sheumack
- R. ClaassensRCR. Claassens
- N.M. WhiteleyNWN.M. Whiteley
- M.H.H. HowdenMHM.H.H. Howden
- 11 February 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 181 (1) , 154-156
Abstract
Robustoxin, the lethal neurotoxin isolated from the venom of the male Sydney funnel–web spider, Atrax robustus, is of unique structural type and physiological mode of action. The primary structure of this 42–residue peptide was determined to be H2N–Cys–Ala–Lys–Lys–Arg–Asn–Trp–Cys–Gly–Lys10–Asn–Glu–Asp–Cys–Cys–Cys–Pro–Met–Lys–Cys20–Ile–Tyr–Ala–Trp–Tyr–Ala–Trp–Tyr–Asn–Gln–Gln–Gly–Ser30–Cys–Gln–Thr–Thr–Ile–Thr–Gly–Leu–Phe–Lys40–Lys–Lys–Cys–H The disposition of disulphide–bridged cysteine residues at both the amino– and carboxy–termini and as a triplet at residues 14–16 appears to have no precedent amongst neurotoxins.Keywords
This publication has 6 references indexed in Scilit:
- The isolation and N-terminal sequence of the lethal neurotoxin from the venom of the male Sydney funnel web spider,Toxicon, 1983
- A gas-liquid solid phase peptide and protein sequenator.Journal of Biological Chemistry, 1981
- Atrax Robustus EnvenomationAnaesthesia and Intensive Care, 1980
- Immobilized carboxypeptidase Y. applications in protein chemistryFEBS Letters, 1976
- Multiple peptide composition of the large and small sub-units of Nicotiana tabacum fraction I protein ascertained by fingerprinting and electrofocusingBiochimica et Biophysica Acta (BBA) - Protein Structure, 1974
- The spectrophotometric determination of tyrosine and tryptophan in proteinsBiochemical Journal, 1946