Stain-free subcellular-resolution astrocyte imaging using third-harmonic generation
- 14 June 2019
- journal article
- research article
- Published by Optica Publishing Group in Optics Letters
- Vol. 44 (12) , 3166-3169
Abstract
We demonstrate stain-free, high-contrast, subcellular-resolution imaging of astroglial cells using epi-detected third-harmonic generation (THG). The astrocyte-imaging capability of THG is verified by colocalizing THG images with fluorescence images of astrocytes expressing a genetically encodable fluorescent reporter. We show that THG imaging with an optimized point-spread function can reliably detect significant subcellular features of astrocytes, including cell nuclei, as well as the soma shape and boundaries.Keywords
Funding Information
- Russian Foundation for Basic Research (17-00-00212, 17-00-00214, 18-29-20031, 18-32-20191, 18-02-40025)
- Russian Science Foundation (18-72-10094, 17-15-01175)
- Welch Foundation (A-1801-20180324)
- Office of Naval Research (00014-16-1-2578)
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