An adaptive excitation source for high-speed multiphoton microscopy
- 2 December 2019
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 17 (2) , 163-166
Abstract
Optical imaging is important for understanding brain function. However, established methods with high spatiotemporal resolution are limited by the potential for laser damage to living tissues. We describe an adaptive femtosecond excitation source that only illuminates the region of interest, which leads to a 30-fold reduction in the power requirement for two- or three-photon imaging of brain activity in awake mice for improved high-speed longitudinal neuroimaging.Keywords
Funding Information
- U.S. Department of Health & Human Services | National Institutes of Health (U01NS090530)
- National Science Foundation (DBI-1707312)
This publication has 21 references indexed in Scilit:
- In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brainNature Methods, 2017
- A large field of view two-photon mesoscope with subcellular resolution for in vivo imagingeLife, 2016
- Simultaneous Denoising, Deconvolution, and Demixing of Calcium Imaging DataNeuron, 2016
- Simultaneous all-optical manipulation and recording of neural circuit activity with cellular resolution in vivoNature Methods, 2014
- In vivo three-photon microscopy of subcortical structures within an intact mouse brainNature Photonics, 2013
- Scanless two-photon excitation of channelrhodopsin-2Nature Methods, 2010
- Imaging Large-Scale Neural Activity with Cellular Resolution in Awake, Mobile MiceNeuron, 2007
- Deep tissue two-photon microscopyNature Methods, 2005
- Nonlinear magic: multiphoton microscopy in the biosciencesNature Biotechnology, 2003
- Two-Photon Laser Scanning Fluorescence MicroscopyScience, 1990