Incorporation of L‐ 75 Se‐cystine in tissue fragments from the matrix of the hoof and the claw — A tool for studying the pathogenesis of laminitis?

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Abstract
Summary: An in vitro method has been designed and used to study the incorporation of 75 Se‐cystine into matrix fragments from hooves and claws of healthy horses and cattle. Tissue fragments from the zone of keratinisation were incubated with L‐ 75 Se‐cystine in a tissue culture medium for 4 to 6 h, during which time there was continuous incorporation of the labelled selenocystine. The incorporation was greatly decreased by adding L‐cystine to the incubation mixture. It is concluded that the incorporation of 75 Se‐cystine depends on the presence of a specific receptor for cystine in the tissue fragments studied. The possible application of the method to studies of the pathogenesis of laminitis is discussed.