Rapid determination of haloperidol and its metabolites in human plasma by HPLC using monolithic silica column and solid‐phase extraction

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Abstract
A rapid, sensitive and reproducible HPLC method was developed and validated for the analysis of haloperidol and its three main metabolites in human plasma. The analysis was carried out on a monolithic silica column (Chromolith Performance RP‐18e, 100 × 4.6 mm). The mobile phase consisted of sodium phosphate (0.1 m, pH 3.5)–acetonitrile (80:20, v/v) at a flow rate of 2.0 mL/min. UV detection at 230 nm was used, with the detection limits of these compounds ranging from 2 to 5 ng. The separation factors of all studied compounds were in the range 2.30–16.32, while the resolution factors were from 1.00 to 5.37. Copyright © 2005 John Wiley & Sons, Ltd.