Concomitant production of different lymphokines in activated T cells

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Abstract
The intracellular accumulation of five different lymphokines in individual cells could be identified by lymphokine‐specific antibodies and an indirect immunofluorescence technique with UV microscopy. When peripheral blood mononuclear cells from one healthy donor were activated in vitro by the T cell mitogen anti‐CD3 monoclonal antibody, the maximal number of cells producing interleukin (IL)2, IL6 or tumor necrosis factor (TNF)−α occurred 6 h after initiation of the cultures, while peak numbers of interferon‐γ, TNF‐β and a second wave of TNF‐α‐synthesizing cells were noted approximately 20 h later. By performing two‐color immunofluorescence studies we observed a variegated production pattern with cells making no, one or several lymphokines simultaneously. All five cytokines accumulated in the Golgi organelle resulting in a very characteristic morphology of the staining with or without additional cytoplasmic immunofluorescence.

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