NITROGENASE CONFINED TO RANDOMLY DISTRIBUTED TRICHOMES IN THE MARINE CYANOBACTERIUM TRICHODESMIUM THIEBAUTII1
Authors:
- 1 April 1991
- journal article
- Published by Wiley in Journal of Phycology
- Vol. 27 (2) , 158-165
Abstract
Nitrogenase reductase (Fe‐protein) was detected in the marine planktonic cyanobacterium Trichodesmium. The molecular weight was about 38 kD, as shown by western blotting using anti ‐Rhodospirillum rubrum nitrogenase reductase antiserum. The enzyme was confined to a limited number (ca. 10–40%) of randomly distributed trichomes in the Trichodesmium colonies, as shown by immunogold localization and transmission electron microscopy. Associated microorganisms had little or no nitrogenase. Nitrogenase showed a diel cycle in localization: present throughout the cytoplasm of cells in N2‐fixing (daytime) colonies but at the periphery of non‐N2‐fixing (nighttime) colonies. This structural arrangement of N2‐fixing trichomes and nitrogenase is novel and different from the previously held paradigm for this and other diazotrophic cyanobacteria.Keywords
This publication has 20 references indexed in Scilit:
- Correlation between nitrogenase and glutamine synthetase expression in the cyanobacterium Anabaena cylindricaPhysiologia Plantarum, 1990
- The Nostoc-Nephroma symbiosis: localization, distribution pattern and levels of key proteins involved in nitrogen and carbon metabolism of the cyanobiontPhysiologia Plantarum, 1989
- Ultrastructure of the nitrogen-fixing, filamentous, nonheterocystous cyanobacterium,Plectonema boryanumProtoplasma, 1989
- Strategy by which nitrogen-fixing unicellular cyanobacteria grow photoautotrophicallyNature, 1986
- Nitrogenase in free-living and symbiotic cyanobacteria: Immunoelectron microscopic localizationFEMS Microbiology Letters, 1986
- Rearrangement of nitrogen fixation genes during heterocyst differentiation in the cyanobacterium AnabaenaNature, 1985
- Nitrogen Fixation in the Marine EnvironmentScience, 1982
- Acetylene reduction (nitrogen fixation) by cyanobacteria grown under alternating light-dark cyclesFEMS Microbiology Letters, 1981
- Acetylene reduction (nitrogen fixation) by cyanobacteria grown under alternating light-dark cyclesFEMS Microbiology Letters, 1981
- Marine Oscillatoria ( Trichodesmium ): Explanation for Aerobic Nitrogen Fixation Without HeterocystsScience, 1976