Electrochemical immobilization of enzymes. Part VI. Microelectrodes for the detection of L-lactate based on flavocytochrome b 2 immobilized in a poly(phenol) film

Authors:
  • PBPhilip N. Bartlett
    Philip N. Bartlett
  • DCDaren J. Caruana
    Daren J. Caruana
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Abstract
The adsorption of flavocytochrome b2 at a 25 µm diameter platinum microelectrode, followed by immobilization in an electrochemically polymerized phenol film, was found to be a reproducible method for the fabrication of microelectrodes responsive to L-lactate. Fabrication of enzyme microelectrodes in this manner is simple and produces electrodes that respond rapidly to L-lactate. The immobilization in the poly(phenol) film has no effect on the specificity of the enzyme for L-lactate over β-chlorolactate and reduces the direct electrochemical interference from uric acid, acetomidophenol and ascorbate. The stability of the resulting enzyme electrode is discussed.
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