Molecular identification of Eimeria tenella in broiler chickens in Kalyoubia governorate and evaluation of different strategies for control cecal coccidiosis.

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Abstract
Ceacal coccidiosis one of Nine Eimeria species affecting chickens and results in severe economic losses. Sixty-seven GIT sample were collected from broiler flocks showing bloody diarrhea in Kalyoubia governorate. 88% of collected samples were found to be positive for cecal coccidiosis after using microscopical and molecular identification. Different control strategies as diclazuril, vaccination (coccivac B® and isolated strain as field strain vaccine) and the aqueous extract of neem plant (Azadirachta indica) were used to control cecal coccidiosis in experimentally infected broiler chicks. The results showed that the diclazuril was the best protection method as there was a significant improvement in performance parameters, significant drop in oocyst shedding, dropping scoring, lesion scoring and cecal mucosal scraping scoring. Also, there was minimal histopathological alteration in the cecum of infected broilers in comparison with other treatments. The neem extract treated group also recorded an improvement in the aforementioned parameters in comparison with the vaccinated infected birds. The vaccination of birds using isolated strain achieved better protection against coccidia infection than the imported strain.

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