Multiple cropping in conjunction with various minimum tillage practices is currently a conspicuous agricultural production method in the southeastern U.S. The objective of this study was to determine the influence of 5 years of various tillage practices on the fertility status of an Ultisol continuously double‐cropped to wheat (Triticum aestivum L.) and soybeans (Glycine max L.). The tillage treatments for each fall/spring were: no‐tillage/no‐tillage, harrow/ho‐tillage, conventional tillage/no‐tillage, no‐tillage/conventional tillage, harrow/conventional tillage, conventional tillage/conventional tillage. With no‐tillage, soil pH decreased more rapidly with depth than with conventional tillage treatments. Accumulations of Ca, Mg, P, Mn, and Zn occurred in the surface soil with no‐tillage treatments, but surface soil K was lower with no‐tillage compared to conventional tillage treatments. Organic C and N did not accumulate in the soil surface under no‐tillage to the degree reported in other studies. Results indicate that continuous no‐tillage results in increased nutrient concentrations in the surface soil with a rapid decrease with depth, while conventional tillage resulted in a more homogeneous soil with respect to soil fertility status