Resveratrol Suppresses Annulus Fibrosus Cell Apoptosis through Regulating Oxidative Stress Reaction in an Inflammatory Environment

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Abstract
During disc degeneration, the increase of inflammatory cytokines and decrease of disc cell density are two prominent features. Enhanced inflammatory reaction contributes to disc annulus fibrosus (AF) cell apoptosis. In this study, we investigated whether resveratrol can suppress AF cell apoptosis in an inflammatory environment. Rat disc AF cells were cultured in medium with or without tumor necrosis factor‐α (TNF‐α). Resveratrol was added along with the culture medium supplemented with TNF‐α. Caspase‐3 activity, cell apoptosis ratio, expression of apoptosis‐associated molecules (Bcl‐2, Bax, caspase‐3, cleaved PARP, and cleaved caspase‐3), reactive oxygen species (ROS) content, and the total superoxide dismutase (SOD) activity were measured. Our results showed that TNF‐α significantly increased caspase‐3 activity and AF cell apoptosis ratio and upregulated gene/protein expression of Bax, caspase‐3, cleaved caspase‐3, and cleaved PARP, whereas it downregulated the expression of Bcl‐2. Moreover, TNF‐α significantly increased ROS content but decreased the total SOD activity. Further analysis demonstrated that resveratrol partly attenuated the effects of TNF‐α on AF cell apoptosis‐associated parameters, decreased ROS content, and increased the total SOD activity in the AF cells treated with TNF‐α. In conclusion, resveratrol attenuates inflammatory cytokine TNF‐α‐induced AF cell apoptosis through regulating oxidative stress reaction in vitro. This study sheds a new light on the protective role of resveratrol in alleviating disc degeneration.

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