Effect of Oxygen on the Induction of Tryptophan Hydroxylase by Adrenergic Agents in Organ Cultures of Rat Pineal Glands
Authors:
- 1 April 1984
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 42 (4) , 1183-1185
Abstract
Rat pineal glands, cultured under 95% air and 5% CO2, lost 50% of their tryptophan hydroxylase activity within 5 h. This loss was accelerated by the addition of cycloheximide or puromycin to the medium. Activity was, however, largely maintained in 95% O2 and 5% CO2. Under these conditions, L-noradrenaline (100 microM), L-isoproterenol (10 microM), and dibutyryl cyclic AMP (1 mM) induced enzyme activity. They failed to do so when 95% air and 5% CO2 was used. Noradrenaline induced serotonin N-acetyltransferase activity with either atmosphere.Keywords
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